Cd- and Zn-NagA were utilised to deal with the incidence of direct interactions of the carbonyl oxygen/sulfur along with the metal ion via a comparison from the kinetic constants for N
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The constructions of your compounds tested as substrates for NagA are introduced in Plan 6. The substitution of sulfur for oxygen can be utilized at the side of really hard and gentle metals to probe the probable for metallic-ligand conversation throughout the activation of electrophilic reactions (24, twenty five).
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Residues Q59 and N61 ended up mutated jointly into a set of histidine residues to make an HxH motif that is analogous to that located link naga26 in NagA from B. subtilis
There is no evidence to suggest that Glu-131 performs any function in catalysis in addition to to help link alternatif naga26 you coordinate the single divalent cation. The enzyme is level restricted via the cleavage on the amide bond.